Updated on 2019/04/17

写真a

 
SHIMURA Kiyohito
 
Organization
School of Medicine (Life Sciences and Social Medicine) Department of Cellular and Integrative Physiology Postdoctoral Researcher
Title
Postdoctoral Researcher
Contact information
メールアドレス

Degree

  • Ph. D. (Pharmaceutical Science) ( 1985.3   Hokkaido University )

Research Interests

  • capillary electrophoresis, isoelectric focusing, bioaffinity, molecular interaction, immunoassay

Research Areas

  • Nanotechnology/Materials / Analytical chemistry  / Bioanalytical Chemistry

  • Others / Others  / Capillary Electrophoresis

Education

  • Hokkaido University   Graduate School, Division of Pharmaceutical Sciences   Pharmaceutical Biochemistry

    - 1979.3

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    Country: Japan

  • Graduate School of Hokkaido University   Faculty of Pharmaceutical Sciences   Biological Chemistry

    - 1979.3

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    Country: Japan

Research History

  • Fukushima Medical University   School of Medicine (Life Sciences and Social Medicine) Department of Cellular and Integrative Physiology   Postdoctoral Researcher

    2019.4 - Now

  • Fukushima Medical University   School of Medicine (Human and Natural Sciences) Department of Natural Sciences (The Section of Advanced Chemistry)   Professor

    2017.2 - 2019.3

  • Professor

    2010.4 - Now

Professional Memberships

  • Japanese Electrophoresis Society

  • The Division of Electrophoresis, The Japan Society for Analytical Chemistry

  • その他

Committee Memberships

  • 日本電気泳動学会   理事  

    2018.4 - 2020.3   

  • The Division of Electrophoresis, The Japan Society for Analytical Chemistry   The member of standing committee  

    2018.3 - 2020.2   

  •   Editorial Board of the journal "Electrophoresis (Wiley-VCH, Weinheim)"  

       

Papers

  • The prospect of direct coupling of immunoaffinity capture and capillary isoelectric focusing in analysis of protein drugs

    Shimura Kiyohito

    Electrophoresis Letters   62 ( 1 )   19 - 21   2018.7( ISSN:2189-2628

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI : 10.2198/electroph.62.19

  • バイオ医薬÷電気泳動:電気泳動でみるバイオ医薬品の特性 免疫アフィニティー捕捉とキャピラリー等電点電気泳動を一体化した分析法のタンパク質医薬分析における可能性

    志村 清仁

    電気泳動   62 ( 1 )   19 - 21   2018.7( ISSN:2189-2628

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    Language:English   Publishing type:Research paper (scientific journal)  

  • Cesium reversibly suppresses HeLa cell proliferation by inhibiting cellular metabolism

    Kobayashi Daisuke, Kakinouchi Kei, Nagae Tomoki, Nagai Toshihiko, Shimura Kiyohito, Hazama Akihiro

    FEBS Letters   591 ( 5 )   718 - 727   2017.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI : 10.1002/1873-3468.12579

  • Capillary isoelectric focusing after sample enrichment with immunoaffinity chromatography in a single capillary

    Kiyohito Shimura and Toshihiko Nagai

    Sci. Rep.   6   39221   2016.12( ISSN:2045-2322

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI : 10.1038/srep39221

    PubMed

    Other Link : http://www.nature.com/articles/srep39221

  • Direct coupling of immobilized metal ion affinity chromatography and capillary isoelectric focusing in a single capillary

    Kiyohito Shimura and Toshihiko Nagai

    J. Electrophoresis   59 ( 1 )   7 - 17   2015.12

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    Language:English   Publishing type:Research paper (other academic)  

    DOI : 10.2198/jelectroph.59.7

    Other Link : https://www.jstage.jst.go.jp/article/jelectroph/59/1/59_7/_article

  • Pattern analysis of post-translational modification using APCE

    Kiyohito Shimura

    Electrophoresis Letters   58 ( 2 )   74 - 76   2014.10( ISSN:0031-9082

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    Language:Japanese   Publishing type:Research paper (other academic)  

    DOI : 10.2198/sbk.58.74

    Other Link : https://www.jstage.jst.go.jp/browse/sbk/-char/ja/

  • 【最新の電気泳動技術】 アフィニティープローブキャピラリー電気泳動による翻訳後修飾パターン分析

    志村 清仁

    生物物理化学   58 ( 2 )   74 - 76   2014.10( ISSN:0031-9082

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    Language:English   Publishing type:Research paper (scientific journal)  

  • Affinity probe capillary electrophoresis of insulin using a fluorescence-labeled recombinant Fab as an affinity probe

    Kiyohito Shimura, Ken-ichi Kasai

    Electrophoresis   35 ( 6 )   840 - 845   2014.3

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    Language:English   Publishing type:Research paper (other academic)  

    DOI : 10.1002/elps.201300464

  • Estimation of the deamidation rates of major deamidation sites in a Fab fragment of mouse IgG1-kappa by capillary isoelectric focusing of mutated Fab fragments

    Shimura, Kiyohito; Hoshino, Makoto; Kamiya, Kei-ichiro; Enomoto, Manabu; Hisada, Sunao; Matsumoto, Hiroyuki; Novotny, Mark; Kasai, Ken-ichi

    Anal. Chem.   85 ( 3 )   1705 - 10   2013.1

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    Language:English   Publishing type:Research paper (other academic)  

    DOI : 10.1021/ac3033992

    PubMed

  • Quantitative evaluation of lectin-reactive glycoforms of alpha(1) -acid glycoprotein using lectin affinity capillary electrophoresis with fluorescence detection

    Shimura, K., Tamura, M., Toda, T., Yazawa, S., Kasai, K.

    Electrophoresis   32 ( 16 )   2188 - 2193   2011.6( ISSN:0173-0835

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    Language:English   Publishing type:Research paper (other academic)  

    DOI : 10.1002/elps.201100146

    PubMed

    Other Link : http://onlinelibrary.wiley.com/doi/10.1002/elps.201100146/abstract;jsessionid=A692619B8E0AEC2F3A685D

  • Two-step perpendicular free-solution isoelectric focusing in a microchamber array chip

    Ishibashi, R., Kitamori, T. and Shimura, K.

    Lab Chip   10 ( 19 )   2628 - 2631   2010.10

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    Language:English   Publishing type:Research paper (other academic)  

    DOI : 10.1039/c004176a

    Other Link : http://pubs.rsc.org/en/Content/ArticleLanding/2010/LC/c004176a

  • Charge heterogeneity of a therapeutic monoclonal antibody conjugated with a cytotoxic antitumor antibiotic, calicheamicin

    Maeda, E., Urakami, K., Shimura, K., Kinoshita, M., Kakehi, K.

    J. Chromatogr. A   1217 ( 45 )   7164 - 7171   2010.9

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    Language:English   Publishing type:Research paper (other academic)  

    DOI : 10.1016/j.chroma.2010.09.022

    Other Link : http://www.sciencedirect.com/science/article/pii/S0021967310012422

  • Micro OS-ELISA: Rapid noncompetitive detection of a small biomarker peptide by open-sandwich enzyme-linked immunosorbent assay (OS-ELISA) integrated into microfluidic device

    Ihara, M., Yoshikawa, A., Wu, Y., Takahashi, H., Mawatari, K., Shimura, K., Sato, K., Kitamori, T. and Ueda, H.

    Lab. Chip   10 ( 1 )   92 - 100   2010.1

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    Language:English   Publishing type:Research paper (other academic)  

    DOI : 10.1039/B915516C

    Other Link : http://www.rsc.org/publishing/journals/LC/article.asp?doi=b915516c

  • A capillary holder for scanning detection of capillary isoelectric focusing with laser-induced fluorescence

    Takahashi, K., Maruo, Y., Kitamori, T. and Shimura, K.

    J. Sep. Sci.   32 ( 3 )   394 - 398   2009.1( ISSN:1615-9314

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    Language:English   Publishing type:Research paper (other academic)  

    DOI : 10.1002/jssc.200800609

    Other Link : http://www3.interscience.wiley.com/journal/121637732/abstract

  • Recent advances in isoelectric focusing in capillary tubes and microchips

    Shimura, K.

    Electrophoresis   30 ( 1 )   11 - 28   2009.1( ISSN:1522-2683

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    Language:English   Publishing type:Research paper (other academic)  

    DOI : 10.1002/elps.200800615

    Other Link : http://www3.interscience.wiley.com/journal/121581652/abstract

  • Isoelectric Focusing in a Microfluidically Defined Electrophoresis Channel

    Shimura, K., Takahashi, K., Koyama, Y., Sato, K. and Kitamori, T

    Anal. Chem.   80 ( 10 )   3818 - 3823   2008.5

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    Language:English   Publishing type:Research paper (other academic)  

    DOI : 10.1021/ac8000594

  • Pesticide Analysis by MEKC on a Micro¬chip with Hydrodynamic Injection from Or¬ganic Extract

    Smirnova, A., Shimura, K., Hibara, A., Proskurnin, M. and Kitamori, T.

    J. Sep. Sci.   31 ( 5 )   904 - 908   2008.3

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    Language:English   Publishing type:Research paper (other academic)  

  • Focusing Separation Methods

    Shimura, K.

    Bunseki   12   659 - 660   2007.12

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    Language:Japanese   Publishing type:Research paper (other academic)  

  • On-chip Connector Valve for Immunoaffinity Chromatography in a Microfluidic Chip

    Shimura, K., Koyama, Y., Sato, K. and Kitamori, T

    J. Sep. Sci.   30 ( 10 )   1477 - 1481   2007.7

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    Language:English   Publishing type:Research paper (other academic)  

    DOI : 10.1002/jssc.200700015

  • Highly Sensitive Detection of Non-Labeled Peptides Using UV Excitation Thermal Lens Microscope/Liquid Chromatography

    Hiki, S., Tokeshi, M., Kakuta, M., Mawatari, K., Kikutani, Y., Sato, K., Hibara, A., Shimura, K., Uchida, N. and Kitamori, T.

    Bunseki Kagaku   56 ( 1 )   1 - 7   2007.1

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    Language:Japanese   Publishing type:Research paper (other academic)  

  • Application of a Micro Multiphase Laminar Flow on a Microchip for Extraction and De¬termination of Derivatized Carbamate Pesticides

    Smirnova, A., Shimura, K., Hibara, A., Proskurnin, M. and Kitamori, T.

    Anal. Sci.   23 ( 1 )   103 - 107   2007.1

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    Language:English   Publishing type:Research paper (other academic)  

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Books

  • Capillary Electromigration Separation Methods

    Colin F. Poole(Capillary Isoelectric Focusing)

    Elsevier  2018.4  ( ISBN:9780128093757

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    Responsible for pages:167-187   Language:English   Book type:Scholarly book

    Authors name of the chapter and paper which it took charge of:Kiyohito Shimura   Publication place:Amsterdam  

    ASIN

    Other Link: https://www.amazon.co.jp/Capillary-Electromigration-Separation-Methods-Handbooks-ebook/dp/B07C7V1N4S/ref=sr_1_fkmr0_1?ie=UTF8&qid=1532060713&sr=8-1-fkmr0&keywords=Capillary+Electromigration+Separation+Methods+1st+Edition

  • Recent development of capillary electrophoresis and microchip electrophoresis

    Shimura, K.

    Japanese Electrophoresis Society  2008.9 

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    Responsible for pages:89-176   Language:Japanese  

    Authors name of the chapter and paper which it took charge of:Shimura, K. (editor)   Publication place:  

  • Uh-huh! Q & A's in Protein Research

    Toda, T., Hirano, H., Nakamura, K.(What is "affinophoresis"?)

    Yodosya  2005.10  ( ISBN:9784897064888

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    Responsible for pages:159-160   Language:Japanese  

    Authors name of the chapter and paper which it took charge of:Shimura, K.   Publication place:Tokyo  

  • The front edge of analytical methods, from scientific engineering to nano-bio

    Umezawa, Y., Sawada, T., Terabe, S.(Gel electrophoresis)

    NTS  2004.12  ( ISBN:4-86043-067-0

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    Responsible for pages:41-51   Language:Japanese  

    Authors name of the chapter and paper which it took charge of:Shimura, K.   Publication place:Tokyo  

  • Principles of Biochemistry (Horton, Moran, Ochs, Rawn, Scrimgeour), Third Edition

    (Chapter 11-14)

    Tokyo Kagaku Dojin  2003.9  ( ISBN:4-8079-0575-9

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    Responsible for pages:251-339   Language:Japanese  

    Authors name of the chapter and paper which it took charge of:Emori, Y., Kawasaki, H., Shimura, K., Soukawa, J. Suzuki, K., Kasai, K., Soukawa, Y.   Publication place:Tokyo  

  • Recognition of Carbohydrates in Biological Systems, Part A, General Procedures, Methods in Enzymology, vol. 362

    Lee, R. T., Lee, Y. C.(Analysis of Lectin-Carbohydrate Interactions by Capillary Affinophoresis)

    Academic Press  2003  ( ISBN:0-12-182265-6

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    Responsible for pages:398-417   Language:English  

    Authors name of the chapter and paper which it took charge of:Shimura, K. and Kasai, K.   Publication place:San Diego  

  • Saishin Denkieidou Jikkennhou (Principles and Applications of Electrophoresis

    Japanese Electrophoresis Society(Affinophoresis)

    Ishiyaku Publishers Inc.  1999.2  ( ISBN:4-263-22230-X

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    Responsible for pages:165-170   Language:Japanese  

    Authors name of the chapter and paper which it took charge of:Shimura, K.   Publication place:Tokyo  

  • Analytical and Preparative Separation Methods of Biomacromolecules

    Aboul-Enein, H.Y.(Determination of Affinity Constant of Lectins for Sugars by Affinity Capillary Electrophoresis)

    Marcel Dekker, Inc.  1999  ( ISBN:0-8247-1996-4

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    Responsible for pages:187-201   Language:English  

    Authors name of the chapter and paper which it took charge of:Shimura, K. and Kasai, K.   Publication place:New York  

  • Principles of Biochemistry (Horton, Moran, Ochs, Rawn, Scrimgeour), Second Edition

    (Chapter 12-15)

    Tokyo Kagaku Dojin  1998.4  ( ISBN:4-8079-0478-7

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    Responsible for pages:233-312   Language:Japanese  

    Authors name of the chapter and paper which it took charge of:Emori, Y., Kawasaki, H., Shimura, K., Soukawa, J. Suzuki, K., Kasai, K., Soukawa, Y.   Publication place:Tokyo  

  • Quantitative Analysis of Biospecific Interactions

    Lundahl, P., Lundqvist, A. and Greijer, E.(Determination of Affinity Constants by Capillary Electrophoresis)

    Harwood Academic Publishers  1998  ( ISBN:90-5702-378-4

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    Responsible for pages:15-34   Language:English  

    Authors name of the chapter and paper which it took charge of:Heegaard, N. H. H. and Shimura, K.   Publication place:Amsterdam  

  • High Resolution Separation and Analysis of Biological Macromolecules, Rart B, Ap-plications, Methods in Enzymology, vol. 271

    Karger, B. L. and Hancock, W. S.(Affinophoresis: Selective Electrophoretic Separation of Proteins by using a Specific Carrier)

    Academic Press  1996  ( ISBN:0-12-182172-2

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    Responsible for pages:203-218   Language:English  

    Authors name of the chapter and paper which it took charge of:Shimura, K. and Kasai, K.   Publication place:San Diego  

  • Methods of Cell Separation (P. T. Sharpe)

    Tokyo Kagaku Dojin  1991.11  ( ISBN:4-8079-1152-X

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    Responsible for pages:全部   Language:Japanese  

    Authors name of the chapter and paper which it took charge of:Shimura, K. and Kasai, K.   Publication place:Tokyo  

  • The New Experimental Course for Biochemistry, Vol. 1, "Protein I"

    Japanese Biochemical Society(Chapter 4, Detection of Protein; Chapter 5, Determination of Protein)

    Tokyo Kagaku Dojin  1990.2  ( ISBN:4-8079-1047-7

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    Responsible for pages:85-107   Language:Japanese  

    Authors name of the chapter and paper which it took charge of:Shimura, K. and Kasai, K.   Publication place:Tokyo  

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Presentations

  • Capillary isoelectric focusing coupled with immunoaffinity: Two micro platforms for analysis of charge variants produced by post translational modifications

    MSB 2018: The 34th International Symposium on Microscale Separation and Bioanalysis  

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    Event date: 2018.2

  • Immunoelectrophoresis instrument for new bio-markers

    New Technology Presenting Meetings  

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    Event date: 2017.12

  • The issues and solutions in the analysis of charge variants of erythropoietin by APCIEF

    The 37th Symposium on Capillary Electrophoresis  

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    Event date: 2017.11

  • Optimization of isoelectric point-variants analysis of erythropoietin by APCIEF

    The 68th general meeting of Japanese Electrophoresis Society  

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    Event date: 2017.11

  • Investigation of IEF conditions with ultra violet absorption detection using PDMA-coated capillaries

    The 68th general meeting of Japanese Electrophoresis Society  

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    Event date: 2017.11

  • Direct coupling of immune extraction and capillary isoelectric focusing in analysis of protein drugs

    The 66th Annual Meeting of the Japan Society for Analytical Chemistry  

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    Event date: 2017.9

  • The prospect of direct coupling of immune extraction and capillary isoelectric focusing in analysis of protein drugs

    The 67th Symposium of Japanese Electrophoresis Society: Characterization of Biologics by Electrophoresis  

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    Event date: 2017.6

  • Direct coupling of immunomagnetic separation and capillary isoelectric focusing

    The 36th Symposium on Capillary Electrophoresis (SCE 2016)  

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    Event date: 2016.11

  • Analysis of isoelectric pattern of erythropoietin using a unified capillary device

    The 67th General Meeting of Japanese Electrophoresis Society  

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    Event date: 2016.8

  • Direct coupling of immunoaffinity column and capillary isoelectric focusing -The third report, The use of a milder anode solution.

    The 67th General Meeting of Japanese Electrophoresis Society  

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    Event date: 2016.8

  • Direct Coupling of Immunoaffinity Chromatography and Capillary Isoelectric Focusing in a Unified Capillary Device

    BIT's 8th Annual International Congress of Antibodies-2016  

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    Event date: 2016.4

  • Determination of a Charge-variant Pattern of Recombinant Erythropoetin using a Unified Affinity Chromatography-CIEF Device

    32nd International Symposium on Microscale Separation and Bioanalysis (MSB 2016)  

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    Event date: 2016.4

  • Determination of a pI pattern of recombinant erythropoietin with APCE

    The 35th Symposium on Capillary Electrophoresis (SCE 2015)  

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    Event date: 2015.11

  • Direct coupling of antibody affinity column and capillary isoelectric focusing -The second report, The stability of antibody column in repeated use

    The 66th General Meeting of the Japanese Electrophoresis Society  

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    Event date: 2015.9

  • Electrophoresis Instrument for Direct Coupling of Affinity Chromatography and Capillary Isoelectric Focusing

    The 34th Symposium on Capillary Electrophoresis (SCE 2014)  

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    Event date: 2014.12

  • On-line Coupling of Immunoaffinity Chromatography and CIEF in a Single Capillary

    14th Asia-Pacific International Symposium on Microscale Separations and Analysis  

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    Event date: 2014.12

  • On-line coupling of Anti-E-tag antibody-immobilized column and capillary isoelectric focusing

    The 65th Annual Meeting of Japanese Electrophoresis Society  

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    Event date: 2014.10

  • Pattern analysis of post-translational modifications using APCE

    The 65th Annual Meeting of Japanese Electrophoresis Society, Symposium 2 "The current electrophoresis technologies"  

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    Event date: 2014.10

  • 金属キレートクロマトグラフィーとキャピラリー等電点電気泳動のオンライン結合

    第74回分析化学討論会  

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    Event date: 2014.5

  • DIRECT COUPLING OF AFFINITY CHROMATOGRAPHY AND CAPILLARY ISOELECTRIC FOCUSING IN A SINGLE CAPILLARY

    30th International Sumposium on MicroScale Bioseparations (MSB 2014)  

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    Event date: 2014.5

  • 翻訳後修飾パターン解析に向けたアフィニティープローブCE (APCE) の開発

    「分離機能とセンシング機能の化学」セミナー 2014  

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    Event date: 2014.3

  • Affinity chromatography of His-tagged protein in a capillary

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    Event date: 2013.11

  • Capillary electrophoresis - To achieve real high-sensitivity and removal of interfering components -

    The 64th General Meeting of Japanese Electrophoresis Society  

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    Event date: 2013.11

  • Sample pre-treatment for capillary isoelectric focusing by using capillary inner wall as a solid-phase support

    The 33rd Symposium on Capillary Electrophoresis  

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    Event date: 2013.11

  • キャピラリー電気泳動技術 ? 実質感度の向上と妨害成分の除去を目指して ?

    第64回 日本電気泳動学会総会  

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    Event date: 2013

  • APCE for the Analysis of Protein Isoforms in Biological Fluids

    SCE 2012, The 32nd Symposium on Capillary Electrophoresis  

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    Event date: 2012.11

  • Inner wall-coated capillary for electrophoretic analysis of ions in cells, The 2nd report

    The 63rd General Meeting of the Japanese Electrophoresis Society  

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    Event date: 2012.8

  • Recent Advance in APCE

    The 62th Symposium of Japanese Electrophoresis Society  

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    Event date: 2012.5

  • Formation of soluble fragments of an insoluble protein and their detection by APCE

    The 62nd Annual General Meeting of the Japanese Electrophoresis Society  

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    Event date: 2011.11

  • Inner wall coated capillary for electrophoretic analysis of ions in cells

    The 62nd Annual General Meeting of the Japanese Electrophoresis Society  

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    Event date: 2011.11

  • APCE of soluble peptides derived from a protein of low solubility

    The 31st Symposium on Capillary Electrophoresis  

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    Event date: 2011.11

  • For the new developments of capillary electrophoresis

    The 61th annual meeting of Japanese Electrophoresis Society  

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    Event date: 2010.9

  • APCE of Insulin using a Recombinant Antibody

    29th Symposium on Capillary Electrophoresis  

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    Event date: 2009.11

  • Affinity Probe Capillary Electrophoresis of Insulin using a Recombinant Affinity Probe

    60th General Meeting of the Japanese Electrophoresis Society  

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    Event date: 2009.9

  • Development of Electrophoresis ~Coming Across with Nano Technologies~

    59th Symposium of the Japanese Electrophoresis Society  

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    Event date: 2009.5

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Industrial property rights

  • 分離分析用キャピラリーデバイス、分離分析用マイクロ流体チップ、タンパク質又はペプチド分析方法、電気泳動装置、及び分離分析用マイクロ流体チップ電気泳動装置

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    Property type:Patent

    Application no:PCT/JP2014/077340  Date applied:2014.10

  • CAPILLARY DEVICE FOR SEPARATION AND ANALYSIS, MICROFLUIDIC CHIP FOR SEPARATION AND ANALYSIS, ANALYSIS METHOD FOR PROTEINS OR PEPTIDES, ELECTROPHORESIS INSTRUMENT, AND MICROFLUIDIC CHIP ELECTROPHORESIS INSTRUMENT FOR SEPARATION AND ANALYSIS

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    Property type:Patent

    Application no:PCT/JP2014/077340  Date applied:2014.10

    Patent/Registration no:U.S. Pat. No. 9927399  Date registered:2018.3  Date issued:2018.3

Awards

  • International-Activity Promotion Award

    2009.9  

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    Country:Japan

  • Kodama Award (Japanese Electrophoresis Society)

    1997.10  

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    Country:Japan

Research Projects

  • 電気泳動による新しい免疫応答マーカーの開発(T細胞受容体クローナリティアッセイ)

    2017.4 - 2019.3

  • Mod-form pattern analysis by on-line coupling of immunoextraction and capillary isoelectric focusing

    2014.4 - 2017.3

    Kiyohito Shimura, Toshihiko Nagai

  • Development of APCE method for the analysis of post-administration isoforms of protein drugs in administered patients

    2013.4 - 2014.3

    Shimura, Kiyohito, Nagai, Toshihiko

  • Development of a rapid, precise and easy test for EPO doping

    2011.12 - 2012.7

    SHIMURA, Kiyohito, NAGAI, Toshihiko