Work Chief Such as Four Alkyl Lead

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Organic Solvent Work Chief Person

  20190417

Gamma Ray Penetration Photography Work Chief Person

  20130813

X-ray Work Chief Person

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High School Teacher Specialization License

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First Kind of High School Teacher License

  19990325

Identification and functional and spectral characterization of a globin-coupled histidine kinase from Anaeromyxobacter sp. Fw109-5

Kitanishi, K., Kobayashi, K., Uchida, T., Ishimori, K., #HIgarashi, J.#HR, and Shimizu, T.

J. Biol. Chem.   286 ( 41 )   35522 - 35534   2011.8

Phosphorylation of a heme-regulated eukaryotic initiation factor 2α kinase enhances the interaction with heat-shock protein 90 and substantially upregulates kinase activity

Mukai, K., Shimizu, T., and #HIgarashi, J.#HR

Protein Pept. Lett.   18 ( 12 )   1251 - 1257   2011.7

A hydrogen-bonding network formed by the B10-E7-E11 residues of a truncated hemoglobin from Tetrahymena pyriformis is critical for stability of bound oxygen and nitric oxide detoxification

#HIgarashi, J.#HR, Kobayashi, K., and Matsuoka, A.

J. Biol. Inorg. Chem.   16 ( 4 )   599 - 609   2011.5

Autophosphorylation of heme-regulated eukaryotic initiation factor 2α kinase, HRI, and the role of the modification in catalysis

#HIgarashi, J.#HR, Sasaki, T., Kobayashi, N., Yoshioka, S., Matsushita, M., and Shimizu, T.

FEBS J.   278 ( 6 )   918 - 928   2011.4

Heme binding characteristics of the isolated PAS-B domain of mouse Per2, a transcriptional regulatory factor associated with circadian rhythms

Hayasaka, K., Kitanishi, K., #HIgarashi, J.#HR, and Shimizu, T.

Biochim. Biophys. Acta   1814 ( 2 )   326 - 333   2011.2

Important roles of Tyr43 at the putative heme distal side in oxygen recognition and stability of the Fe(II)-O₂ complex of YddV, a globin-coupled heme-based oxygen sensor diguanylate cyclase

Kitanishi, K., Kobayashi, K., Kawamura, Y., Ishigami, I., Ogura, T., Nakajima, K., #HIgarashi, J.#HR, Tanaka, A., and Shimizu, T.

Biochemistry   49 ( 49 )   10381 - 10393   2010.12

Catalysis and oxygen binding of Ec DOS, a heme-based oxygen-sensor enzyme from Escherichia coli

Kobayashi, K., Tanaka, A., Takahashi, H., #HIgarashi, J.#HR, Ishitsuka, Y., Yokota, N., and Shimizu, T.

J. Biochem.   148 ( 6 )   693 - 703   2010.12

ヘムによる時計遺伝子の転写制御

北西健一, #H五十嵐城太郎#HR, 清水透

生物物理   50 ( 1 )   34 - 35   2010.1

The FG loop of a heme-based gas sensor enzyme, Ec DOS, functions in heme binding, autoxidation and catalysis

Ito, S., #HIgarashi, J.#HR, and Shimizu, T.

J. Inorg. Biochem.   103 ( 10 )   1380 - 1385   2009.10

Role of Phe113 at the distal side of the heme domain of an oxygen-sensor (Ec DOS) in the characterization of the heme environment

Ito, S., Araki, Y., Tanaka, A., #HIgarashi, J.#HR, Wada, T., and Shimizu, T.

J. Inorg. Biochem.   103 ( 7 )   989 - 996   2009.7

Crystal structures of constitutive nitric oxide synthases in complex with de novo designed inhibitors

#HIgarashi, J.#HR, Li, H., Jamal, J., Ji, H., Fang, J., Lawton, G., Silverman, R. B., and Poulos, T. L.

J. Med. Chem.   52 ( 7 )   2060 - 2066   2009.3

Selective neuronal nitric oxide synthase inhibitors and the prevention of cerebral palsy

Ji, H., Tan, S., #HIgarashi, J.#HR, Li, H., Derrick, M., Martásek, P., Roman, L., Vásquez-Vivar, J., Poulos, T. L., and Silverman, R. B.

Ann. Neurol.   65 ( 2 )   209 - 217   2009.2

ヘムの移動により機能する生体反応―ヘムセンサータンパク質・酵素とヘム輸送体

#H五十嵐城太郎#HR, 清水透

化学   63 ( 11 )   66 - 67   2008.11

Arg97 at the heme-distal side of the isolated heme-bound PAS domain of a heme-based oxygen sensor from Escherichia coli (Ec DOS) plays critical roles in autoxidation and binding to gases, particularly O₂

Ishitsuka, Y., Araki, Y., Tanaka, A., #HIgarashi, J.#HR, Ito, O., and Shimizu, T.

Biochemistry   47 ( 34 )   8874 - 8884   2008.9

Elucidation of the heme-binding site of heme-regulated eIF2α kinase (HRI) and the role of the regulatory motif in heme sensing by spectroscopic and catalytic studies of mutant proteins

#HIgarashi, J.#HR, Murase, M., Iizuka, A., Pichierri, F., Martinkova, M., and Shimizu, T.

J. Biol. Chem.   283 ( 27 )   18782 - 18791   2008.7

Heme-binding characteristics of the isolated PAS-A domain of mouse Per2, a transcriptional regulatory factor associated with circadian rhythms

Kitanishi, K., #HIgarashi, J.#HR, Hayasaka, K., Hikage, N., Saiful, I., Yamauchi, S., Uchida, T., Ishimori, K., and Shimizu, T.

Biochemistry   47 ( 23 )   6157 - 6168   2008.6

Minimal pharmacophoric elements and fragment hopping, an approach directed at molecular diversity and isozyme selectivity. Design of selective neuronal nitric oxide synthase inhibitors

Ji, H., Stanton, B. Z., #HIgarashi, J.#HR, Li, H., Martásek, P., Roman, L. J., Poulos, T. L., and Silverman, R. B.

J. Am. Chem. Soc.   130 ( 12 )   3900 - 3914   2008.3

The roles of thiolate-heme proteins, other than the P450 cytochromes, in the regulation of heme-sensor proteins

#HIgarashi, J.#HR, Kitanishi, K., Martinkova, M., Murase, M., Iizuka, A., and Shimizu, T.

Acta Chim. Slov.   55 ( 1 )   67 - 74   2008.1

ドメイン間相互作用による酵素活性の制御

#H五十嵐城太郎#HR, 田中敦成, 清水透

生体の科学   58 ( 5 )   354 - 356   2007.10

Identification of Cys385 in the isolated kinase insertion domain of heme-regulated eIF2α kinase (HRI) as the heme axial ligand by site-directed mutagenesis and spectral characterization

Hirai, K., Martinkova, M., #HIgarashi, J.#HR, Saiful, I., Yamauchi, S., El-Mashtoly, S. F., Kitagawa, T., and Shimizu, T.

J. Inorg. Biochem.   101 ( 8 )   1172 - 1179   2007.8

Eukaryotic initiation factor 2α kinase is a nitric oxide-responsive mercury sensor enzyme: potent inhibition of catalysis by the mercury cation and reversal by nitric oxide

Martinkova, M., #HIgarashi, J.#HR, and Shimizu, T.

FEBS Lett.   581 ( 21 )   4109 - 4114   2007.8

Structure-based design and synthesis of N^ω-nitro-L-arginine-containing peptidomimetics as selective inhibitors of neuronal nitric oxide synthase. Displacement of the heme structural water

Seo, J., #HIgarashi, J.#HR, Li, H., Martásek, P., Roman, L. J., Poulos, T. L., and Silverman, R. B.

J. Med. Chem.   50 ( 9 )   2089 - 2099   2007.4

海外の研究室訪問20　カリフォルニア大学ポーラス研へようこそ！

#H五十嵐城太郎#HR

化学   61 ( 9 )   50 - 53   2006.9

Structural studies of constitutive nitric oxide synthases with diatomic ligands bound

Li, H., #HIgarashi, J.#HR, Jamal, J., Yang, W., and Poulos, T. L.

J. Biol. Inorg. Chem.   11 ( 6 )   753 - 768   2006.8

Characterization of heme-regulated eIF2α kinase: roles of the N-terminal domain in the oligomeric state, heme binding, catalysis, and inhibition

Miksanova, M., #HIgarashi, J.#HR, Minami, M., Sagami, I., Yamauchi, S., Kurokawa, H., and Shimizu, T.

Biochemistry   45 ( 32 )   9894 - 9905   2006.8

Spectroscopic and DNA-binding characterization of the isolated heme-bound basic helix-loop-helix-PAS-A domain of neuronal PAS protein 2 (NPAS2), a transcription activator protein associated with circadian rhythms

Mukaiyama, Y., Uchida, T., Sato, E., Sasaki, A., Sato, Y., #HIgarashi, J.#HR, Kurokawa, H., Sagami, I., Kitagawa, T., and Shimizu, T.

FEBS J.   273 ( 11 )   2528 - 2539   2006.6

Critical roles of Leu99 and Leu115 at the heme distal side in auto-oxidation and the redox potential of a heme-regulated phosphodiesterase from Escherichia coli

Yokota, N., Araki, Y., Kurokawa, H., Ito, O., #HIgarashi, J.#HR, and Shimizu, T.

FEBS J.   273 ( 6 )   1210 - 1223   2006.4

Exploring the binding conformations of bulkier dipeptide amide inhibitors in constitutive nitric oxide synthases

Li, H., Flinspach, M. L., #HIgarashi, J.#HR, Jamal, J., Yang, W., Gómez-Vidal, J. A., Litzinger, E. A., Huang, H., Erdal, E. P., Silverman, R. B., and Poulos, T. L.

Biochemistry   44   15222 - 15229   2005.11

Spectroscopic characterization of the isolated heme-bound PAS-B domain of neuronal PAS domain protein 2 associated with circadian rhythms

Koudo, R., Kurokawa, H., Sato, E., #HIgarashi, J.#HR, Uchida, T., Sagami, I., Kitagawa, T., and Shimizu, T.

FEBS J.   272 ( 16 )   4153 - 4162   2005.8

Dual effects of the substrate and pterins on the kinetics of CO binding to neuronal nitric oxide synthase: A laser flash photolysis study

Bengea, S., Araki, Y., Ito, O., #HIgarashi, J.#HR, Kurokawa, H., and Shimizu, T.

Chem. Lett.   34 ( 6 )   752 - 753   2005.6

Redox センサータンパク質, ヘム制御ホスホジエスエラーゼの構造と機能

黒河博文, 吉村-鈴木登紀子, 笹倉由貴江, 渡部美紀, 平田智士, 田口須恵, #H五十嵐城太郎#HR, 佐上郁子, 清水透

生物物理   45 ( 2 )   84 - 89   2005.1（ ISSN:1347-4219 ）

SOUL in mouse eyes is a new hexameric heme-binding protein with characteristic optical absorption, resonance Raman spectral, and heme-binding properties

Sato, E., Sagami, I., Uchida, T., Sato, A., Kitagawa, T., #HIgarashi, J.#HR, and Shimizu, T.

Biochemistry   43 ( 44 )   14189 - 14198   2004.11

Analysis of the kinetics of CO binding to neuronal nitric oxide synthase by flash photolysis: Dual effects of substrates, inhibitors, and tetrahydrobiopterin

Bengea, S., Araki, Y., Ito, O., #HIgarashi, J.#HR, Sagami, I., and Shimizu, T.

J. Inorg. Biochem.   98 ( 7 )   1210 - 1216   2004.7

Activation of heme-regulated eukaryotic initiation factor 2α kinase by nitric oxide is induced by the formation of a five-coordinate NO-heme complex: optical absorption, electron spin resonance, and resonance Raman spectral studies

#HIgarashi, J.#HR, Sato, A., Kitagawa, T., Yoshimura, T., Yamauchi, S., Sagami, I., and Shimizu, T.

J. Biol. Chem.   279 ( 16 )   15752 - 15762   2004.4

Binding of oxygen and carbon monoxide to a heme-regulated phosphodiesterase from Escherichia coli. Kinetics and infrared spectra of the full-length wild-type enzyme, isolated PAS domain, and Met-95 mutants

Taguchi, S., Matsui, T., #HIgarashi, J.#HR, Sasakura, Y., Araki, Y., Ito, O., Sugiyama, S., Sagami, I., and Shimizu, T.

J. Biol. Chem.   279 ( 5 )   3340 - 3347   2004.1

CO binding study of mouse heme-regulated eIF-2α kinase: kinetics and resonance Raman spectra

#HIgarashi, J.#HR, Sato, A., Kitagawa, T., Sagami, I., and Shimizu, T.

Biochim. Biophys. Acta   1650 ( 1-2 )   99 - 104   2003.8

A primitive myoglobin from Tetrahymena pyriformis: Its heme environment, autoxidizability, and genomic DNA structure

Korenaga, S., #HIgarashi, J.#HR, Matsuoka, A., and Shikama, K.

Biochim. Biophys. Acta   1543 ( 1 )   131 - 145   2000.11

酵素利用技術大系

小宮山眞（シトクロムP450）

エヌ・ティー・エス  2010.4  （ ISBN:978-4-86043-271-3 ）

P450の分子生物学　第2版

大村恒雄, 石村巽, 藤井義明（他のヘムーチオレート蛋白質の構造と機能）

講談社  2009.8  （ ISBN:978-4-06-153693-7 ）

組換えヒトヘモグロビンの作製

日本動物学会第90回大阪大会2019  

一酸化窒素，一酸化炭素によるヘムタンパク質の機能調節：翻訳開始因子キナーゼとジグアニル酸シクラーゼについて

第32回生物無機化学夏季セミナー  

Candida norvegensisフラボヘモグロビンの安定性と構造基盤

第91回日本生化学会大会  

グロビン結合型ジグアニル酸シクラーゼDgcOの一酸化炭素結合による構造変化と活性調節機構

第40回日本分子生物学会年会・第90回日本生化学会大会（2017年度生命科学系学会合同年次大会）  

Hoki myoglobin with unusual autoxidation behavior: Comparative analysis of native protein and H60V mutant.

The 22nd International Congress of Zoology and the 87th meeting of Zoological Society of Japan  

酸素センサータンパク質YddVのリガンド識別機構と活性調節

第89回日本生化学会大会  

Ligand sensing mechanism of globin domain of a heme-based oxygen-sensor enzyme, YddV, from Escherichia coli

XIXth International Conference of Oxygen Binding and Sensing Protein  

酸素センサータンパク質 YddV のグロビンドメインの構造解析

日本動物学会平成28年度東北支部大会  

酸素センサータンパク質YddVのグロビンドメインの構造解析

第38回日本分子生物学会年会・第88回日本生化学会大会合同大会（BMB2015）  

Crystal structure of isolated heme-bound globin domain of a heme-based oxygen-sensor, YddV, from Escherichia coli

The 53rd Annual Meeting of the Biophysical Society of Japan  

Stability properties of myoglobin in the Argentinian hoki (Macruronus magellanicus).

The 9th International Congress of Comparative Physiology and Biochemistry  

酸素センサー酵素Ec DOSのM30A変異体における構造変化

第87回日本生化学会大会  

翻訳開始因子キナーゼHRIを介したヘムによる翻訳調節

第86回日本生化学会大会  

イミダゾール結合による酸素センサー酵素Ec DOSの調節機構の解明

第85回日本生化学会大会  

原生生物Tetrahymena pyriformisにみられるヘモグロビンの構造機能解析

第84回日本生化学会大会  

原生生物Tetrahymena pyriformisに見られる短縮型ヘモグロビンの立体構造解析

2011年度福島県生物同好会講演会  

Molecular mechanism of NO-induced catalytic activation of mouse heme-regulated eukaryotic initiation factor 2α kinase, HRI

Pacifichem 2010 Congress  

ヘム調節インヒビター（HRI）のヘム・リン酸化による活性調節機構

第33回日本分子生物学会年会・第83回日本生化学会大会合同大会（BMB2010）  

NO-induced activation of a heme-sensor, eIF2α kinase, in association with binding to cysteine and heme

6th International Conference, Biology, Chemistry, and Therapeutic Applications of Nitric Oxide  

ヘム調節インヒビター （HRI）のヘム・リン酸化による反応制御機構

日本生化学会東北支部第76回例会・シンポジウム  

Heme-regulated eukaryotic initiation factor 2α kinase associated with protein translation

Gordon Research Conference on Protein Cofactors, Radicals and Quinones.  

ヘム調節インヒビター（HRI）の活性調節機構：ヘム結合及びリン酸化について

第82回日本生化学会大会  

Heme-sensing mechanisms of heme-sensor proteins associated with protein synthesis and circadian rhythms

14th International Conference on Biological Inorganic Chemistry (ICBIC14)  

Oxygen stability and crystal structure of Tetrahymena truncated hemoglobin

14th International Conference on Biological Inorganic Chemistry (ICBIC14)  

The heme-binding site of the heme-regulated inhibitor (HRI), and the role of heme regulatory motif in heme sensing

16th International Conference on Cytochrome P450, Biochemistry, Biophysics, Functional Genomics  

The critical role of the thiolate-heme complex in the functioning

16th International Conference on Cytochrome P450, Biochemistry, Biophysics, Functional Genomics  

Cysteine thiolate is the heme sensing site in heme-sensor proteins

22nd International Conference on Coordination and Bioinorganic Chemistry  

ヘム調節インヒビター（HRI）の一酸化窒素による活性調節機構：S-ニトロシル化の検討

第9回日本NO学会学術集会  

Identification of the heme axial ligand of HRI: Role of heme regulatory motifs

第31回日本分子生物学会年会・第80回日本生化学会大会合同大会（BMB2008）  

NO-induced activation of a heme-sensor, eIF2α kinase, in association with binding to cysteine and heme

5th International Conference, Biology, Chemistry, and Therapeutic Applications of Nitric Oxide  

Elucidation of the heme-binding site of heme-regulated eIFα kinase (HRI) and the role of the regulatory motif in heme sensing by spectroscopic and catalytic studies of mutant proteins

Gordon Research Conference on the Chemistry & Biology of Tetrapyrroles  

ヘム調節キナーゼHRIのヘムによる制御

第5回東北大学バイオサイエンスシンポジウム  

ヘム制御真核生物翻訳開始因子2αキナーゼ, HRI, のヘムによる反応制御機構

日本生化学会東北支部第74回例会・シンポジウム  

ヘム制御翻訳開始因子eIF2αキナーゼ (HRI) のNOによる活性調節機構

第8回日本NO学会学術集会  

Oxygen stability and crystal structure of Tetrahymena truncated hemoglobin

第45回日本生物物理学会年会  

Identification of a heme axial ligand of heme-regulated eukaryotic initiation factor 2 alpha kinase (HRI): Role of the Cys-Pro motif in heme regulation

13th International Conference on Biological Inorganic Chemistry (ICBIC13)  

神経型NO合成酵素に対するアイソザイム選択的阻害剤の構造基盤

第7回日本NO学会学術集会  

Exploring the binding conformations of bulkier dipeptide amide inhibitors in constitutive nitric oxide synthases

5th East Asia Biophysics Symposium & 44th Annual Meeting of the Biophysical Society of Japan  

タンパク質翻訳に関わるヘム制御キナーゼのNOによる活性化機構

第5回日本NO学会学術集会  

Heme-regulated eukaryotic initiation factor 2α kinase (HRI) has a thiol-coordinated heme and activation of HRI by nitric oxide was exerted by the formation of a five-coordinate NO-heme complex

7th International Symposium on Cytochrome P450, Biodiversity and Biotechnology  

ヘム制御eIF2αキナーゼ(HRI)のNOによる活性化機構

第26回日本ラジカル学会学術集会  

Nitric oxide activates heme-regulated eukaryotic initiation factor 2α kinase (HRI) by forming a five-coordinate NO-heme complex

3rd International Conference on Biology, Chemistry, and Therapeutic Application on Nitric Oxide  

CO binding study of mouse heme-regulated eIF-2α kinase: Kinetics and resonance Raman spectra

第76回日本生化学会大会  

ヘム制御翻訳開始因子eIF2αキナーゼ（HRI）のヘム周辺構造

第53回錯体化学討論会  

CO binding studies of mouse eIF-2α kinase: Kinetics and resonance Raman spectra

11th International Conference on Biological Inorganic Chemistry (ICBIC11)  

全長型ヘム制御eIF2αキナーゼ（HRI）の活性とヘム周辺構造

第30回生体分子科学討論会  

ヘム制御翻訳開始因子eIF2αキナーゼのヘム結合ドメイン：分光学的特性と軸配位子構造

第75回日本生化学会大会  

ヘム制御翻訳開始因子eIF2αキナーゼのN末端ヘム結合ドメインの役割について

第52回錯体化学討論会  

ヘム制御翻訳開始因子eIF2αキナーゼのヘム結合ドメインの分光学的研究

平成14年度生物物理学会東北支部研究発表会  

Tetrahymenaのヘモグロビン様タンパク質：大腸菌での大量発現系の構築

第71回日本動物学会年会  

Tetrahymenaのヘモグロビン遺伝子にイントロンはあるのか？

第70回日本動物学会年会  

Paramecium caudatum短縮型ヘモグロビンの遺伝子構造と発現系の構築

日本動物学会大会94回山形大会2023  

Paramecium caudatum短縮型ヘモグロビンの遺伝子構造と発現系の構築

2023年度日本動物学会東北支部大会  

Paramecium caudatum短縮型ヘモグロビンの遺伝子構造と発現系の構築

2022年度日本動物学会東北支部大会  

翻訳開始因子キナーゼのヘム結合・リン酸化・分解によるダイナミクス

2009.4 - 2011.3

五十嵐城太郎

蛋白質合成におけるヘムと一酸化窒素のクロストーク：翻訳開始因子キナーゼについて

2007.4 - 2009.3

五十嵐城太郎

環境汚染物質による体内時計の破壊:ヘム-NO-蛋白合成-時計遺伝子のクロストーク

2005.4 - 2010.3

清水透、黒河博文、田中敦成、五十嵐城太郎

環境汚染物質によるポルフィリン症の解析・ヘム合成と蛋白質合成の協奏とテンポ

2002.4 - 2005.3

清水透、黒河博文、五十嵐城太郎、佐上郁子

触媒反応を光スイッチや分子スイッチで制御できる環境修復酵素の構築

2001.4 - 2005.3

清水透、黒河博文、五十嵐城太郎、佐上郁子、松井敏高