JSPS Research fellow (PD), University of Michigan   Fellow

2013.11 - 2014.3

JSPS Research fellow (PD), Kobe University   Fellow

2011.4 - 2013.10

G-COE postdoctoral fellow, Kobe University   Researcher

2008.4 - 2011.3

Tight-junction strand networks and tightness of the epithelial barrier.

Akira C Saito, Tomohito Higashi, Hideki Chiba

Microscopy (Oxford, England)   72 ( 3 )   213 - 225   2023.1（ ISSN:2050-5698 ）

Effects of TAMP family on the tight junction strand network and barrier function in epithelial cells.

Akira C Saito, Chisato Endo, Yugo Fukazawa, Tomohito Higashi, Hideki Chiba

Annals of the New York Academy of Sciences   1517 ( 1 )   234 - 250   2022.11（ ISSN:0077-8923 ）

Claudin‑9 is a novel prognostic biomarker for endometrial cancer.

Endo Y, Sugimoto K, Kobayashi M, Kobayashi Y, Kojima M, Furukawa S, Soeda S, Watanabe T, Higashi AY, Higashi T, Hashimoto Y, Fujimori K, Chiba H

International journal of oncology   61 ( 5 )   2022.11（ ISSN:1019-6439 ）

CLDN15 is a novel diagnostic marker for malignant pleural mesothelioma

Watanabe M., Higashi T., Ozeki K., Higashi A.Y., Sugimoto K., Mine H., Takagi H., Ozaki Y., Muto S., Okabe N., Matsumura Y., Hasegawa T., Shio Y., Suzuki H., Chiba H.

Scientific Reports   11 ( 1 )   2021.12

Claudin-9 constitutes tight junctions of folliculo-stellate cells in the anterior pituitary gland.

Higashi AY, Higashi T, Furuse K, Ozeki K, Furuse M, Chiba H

Scientific reports   11 ( 1 )   21642   2021.11

The extracellular domain of angulin-1 and palmitoylation of its cytoplasmic region are required for angulin-1 assembly at tricellular contacts

Yukako Oda, Taichi Sugawara, Yuko Fukata, Yasushi Izumi, Tetsuhisa Otani, Tomohito Higashi, Masaki Fukata, Mikio Furuse

Journal of Biological Chemistry   295 ( 13 )   4289 - 4302   2020.2（ ISSN:0021-9258 ）

Molecular organization, regulation and function of tricellular junctions.

Tomohito Higashi, Hideki Chiba

Biochimica et biophysica acta. Biomembranes   1862 ( 2 )   183143 - 183143   2020.2（ ISSN:0005-2736 ）

Cell adhesion signals regulate the nuclear receptor activity.

Sugimoto K, Ichikawa-Tomikawa N, Kashiwagi K, Endo C, Tanaka S, Sawada N, Watabe T, Higashi T, Chiba H

Proceedings of the National Academy of Sciences of the United States of America   116 ( 49 )   24600 - 24609   2019.12（ ISSN:0027-8424 ）

Rho Flares Repair Local Tight Junction Leaks.

Rachel E Stephenson, Tomohito Higashi, Ivan S Erofeev, Torey R Arnold, Marcin Leda, Andrew B Goryachev, Ann L Miller

Developmental cell   48 ( 4 )   445 - 459.e5   2019.2（ ISSN:1534-5807 ）

Anillin regulates epithelial cell mechanics by structuring the medial-apical actomyosin network.

Arnold TR, Shawky JH, Stephenson RE, Dinshaw KM, Higashi T, Huq F, Davidson LA, Miller AL.

Elife   8   e39065   2019.1（ ISSN:2050-084X ）

Comprehensive analysis of formin localization in Xenopus epithelial cells.

Tomohito Higashi, Rachel E. Stephenson, Ann L. Miller

Molecular Biology of the Cell   30 ( 1 )   82 - 95   2019.1（ ISSN:1939-4586 ）

Tricellular junctions: how to build junctions at the TRICkiest points of epithelial cells.

Higashi T, Miller AL

Molecular biology of the cell   28 ( 15 )   2023 - 2034   2017.7（ ISSN:1059-1524 ）

Maintenance of the Epithelial Barrier and Remodeling of Cell-Cell Junctions during Cytokinesis.

Higashi T, Arnold TR, Stephenson RE, Dinshaw KM, Miller AL

Current biology : CB   26 ( 14 )   1829 - 1842   2016.7（ ISSN:0960-9822 ）

MgcRacGAP restricts active RhoA at the cytokinetic furrow and both RhoA and Rac1 at cell-cell junctions in epithelial cells.

Breznau EB, Semack AC, Higashi T, Miller AL

Molecular biology of the cell   26 ( 13 )   2439 - 2455   2015.7（ ISSN:1059-1524 ）

Downsloping high-frequency hearing loss due to inner ear tricellular tight junction disruption by a novel ILDR1 mutation in the Ig-like domain.

Kim NK, Higashi T, Lee KY, Kim AR, Kitajiri S, Kim MY, Chang MY, Kim V, Oh SH, Kim D, Furuse M, Park WY, Choi BY

PloS one   10 ( 2 )   e0116931   2015

Deficiency of angulin-2/ILDR1, a tricellular tight junction-associated membrane protein, causes deafness with cochlear hair cell degeneration in mice.

Higashi T, Katsuno T, Kitajiri S, Furuse M

PloS one   10 ( 3 )   e0120674   2015

in vivoでの脳と網膜の内皮細胞の3細胞結合におけるアンギュリン-1/LSRとトリセルリンの局在性(Localization of Angulin-1/LSR and Tricellulin at Tricellular Contacts of Brain and Retinal Endothelial Cells in vivo)

Iwamoto Noriko, Higashi Tomohito, Furuse Mikio

Cell Structure and Function   39 ( 1-2 )   1 - 8   2014.12（ ISSN:0386-7196 ）

Localization of angulin-1/LSR and tricellulin at tricellular contacts of brain and retinal endothelial cells in vivo.

Iwamoto N, Higashi T, Furuse M

Cell structure and function   39 ( 1 )   1 - 8   2014（ ISSN:0386-7196 ）

Molecular organization of tricellular tight junctions.

Furuse M, Izumi Y, Oda Y, Higashi T, Iwamoto N

Tissue barriers   2   e28960   2014（ ISSN:2168-8362 ）

ZO-1 knockout by TALEN-mediated gene targeting in MDCK cells: involvement of ZO-1 in the regulation of cytoskeleton and cell shape.

Tokuda S, Higashi T, Furuse M

PloS one   9 ( 8 )   e104994   2014

A "Tric" to tighten cell-cell junctions in the cochlea for hearing.

Higashi T, Lenz DR, Furuse M, Avraham KB

The Journal of clinical investigation   123 ( 9 )   3712 - 3715   2013.9（ ISSN:0021-9738 ）

Analysis of the 'angulin' proteins LSR, ILDR1 and ILDR2--tricellulin recruitment, epithelial barrier function and implication in deafness pathogenesis.

Higashi T, Tokuda S, Kitajiri S, Masuda S, Nakamura H, Oda Y, Furuse M

Journal of cell science   126 ( Pt 4 )   966 - 977   2013.2（ ISSN:0021-9533 ）

Lipolysis-stimulated lipoprotein receptor: a novel membrane protein of tricellular tight junctions.

Furuse M, Oda Y, Higashi T, Iwamoto N, Masuda S

Annals of the New York Academy of Sciences   1257   54 - 58   2012.6（ ISSN:0077-8923 ）

Impact of platelet reactivity on long-term clinical outcomes and bleeding events in Japanese patients receiving antiplatelet therapy with aspirin.

Yamane K, Ikeda T, Taniguchi R, Watanabe S, Kawato M, Kondo H, Shirakawa R, Higashi T, Toma M, Tabuchi A, Tamura T, Takahashi K, Watanabe H, Yoshikawa Y, Kita T, Kimura T, Horiuchi H

Journal of atherosclerosis and thrombosis   19 ( 12 )   1142 - 1153   2012（ ISSN:1340-3478 ）

A coronary artery disease-associated gene product, JCAD/KIAA1462, is a novel component of endothelial cell-cell junctions.

Akashi M, Higashi T, Masuda S, Komori T, Furuse M

Biochemical and biophysical research communications   413 ( 2 )   224 - 229   2011.9（ ISSN:0006-291X ）

LSR defines cell corners for tricellular tight junction formation in epithelial cells.

Masuda S, Oda Y, Sasaki H, Ikenouchi J, Higashi T, Akashi M, Nishi E, Furuse M

Journal of cell science   124 ( Pt 4 )   548 - 555   2011.2（ ISSN:0021-9533 ）

Flightless-I (Fli-I) regulates the actin assembly activity of diaphanous-related formins (DRFs) Daam1 and mDia1 in cooperation with active Rho GTPase.

Higashi T, Ikeda T, Murakami T, Shirakawa R, Kawato M, Okawa K, Furuse M, Kimura T, Kita T, Horiuchi H

The Journal of biological chemistry   285 ( 21 )   16231 - 16238   2010.5（ ISSN:0021-9258 ）

Tuberous sclerosis tumor suppressor complex-like complexes act as GTPase-activating proteins for Ral GTPases.

Shirakawa R, Fukai S, Kawato M, Higashi T, Kondo H, Ikeda T, Nakayama E, Okawa K, Nureki O, Kimura T, Kita T, Horiuchi H

The Journal of biological chemistry   284 ( 32 )   21580 - 21588   2009.8（ ISSN:0021-9258 ）

Evaluation of the antiplatelet effects of cilostazol, a phosphodiesterase 3 inhibitor, by VASP phosphorylation and platelet aggregation.

Yamamoto H, Takahashi K, Watanabe H, Yoshikawa Y, Shirakawa R, Higashi T, Kawato M, Ikeda T, Tabuchi A, Morimoto T, Kita T, Horiuchi H

Circulation journal : official journal of the Japanese Circulation Society   72 ( 11 )   1844 - 1851   2008.11（ ISSN:1346-9843 ）

Biochemical characterization of the Rho GTPase-regulated actin assembly by diaphanous-related formins, mDia1 and Daam1, in platelets.

Higashi T, Ikeda T, Shirakawa R, Kondo H, Kawato M, Horiguchi M, Okuda T, Okawa K, Fukai S, Nureki O, Kita T, Horiuchi H

The Journal of biological chemistry   283 ( 13 )   8746 - 8755   2008.3（ ISSN:0021-9258 ）

Regulation of platelet dense granule secretion by the Ral GTPase-exocyst pathway.

Kawato M, Shirakawa R, Kondo H, Higashi T, Ikeda T, Okawa K, Fukai S, Nureki O, Kita T, Horiuchi H

The Journal of biological chemistry   283 ( 1 )   166 - 174   2008.1（ ISSN:0021-9258 ）

Crystal structure of human DAAM1 formin homology 2 domain.

Yamashita M, Higashi T, Suetsugu S, Sato Y, Ikeda T, Shirakawa R, Kita T, Takenawa T, Horiuchi H, Fukai S, Nureki O

Genes to cells : devoted to molecular & cellular mechanisms   12 ( 11 )   1255 - 1265   2007.11（ ISSN:1356-9597 ）

Constitutive GDP/GTP exchange and secretion-dependent GTP hydrolysis activity for Rab27 in platelets.

Kondo H, Shirakawa R, Higashi T, Kawato M, Fukuda M, Kita T, Horiuchi H

The Journal of biological chemistry   281 ( 39 )   28657 - 28665   2006.9（ ISSN:0021-9258 ）

Purification and functional analysis of a Rab27 effector munc 13-4 using a semi-intact platelet dense-granule secretion assay.

Shirakawa R, Higashi T, Kondo H, Yoshioka A, Kita T, Horiuchi H

Methods in enzymology   403   778 - 788   2005（ ISSN:0076-6879 ）

Direct demonstration of involvement of the adaptor protein ShcA in the regulation of Ca2+-induced platelet aggregation.

Higashi T, Yoshioka A, Shirakawa R, Tabuchi A, Nishioka H, Kita T, Horiuchi H

Biochemical and biophysical research communications   322 ( 2 )   700 - 704   2004.9（ ISSN:0006-291X ）

Munc13-4 is a GTP-Rab27-binding protein regulating dense core granule secretion in platelets.

Shirakawa R, Higashi T, Tabuchi A, Yoshioka A, Nishioka H, Fukuda M, Kita T, Horiuchi H

The Journal of biological chemistry   279 ( 11 )   10730 - 10737   2004.3（ ISSN:0021-9258 ）

Direct demonstration of involvement of protein kinase Calpha in the Ca2+-induced platelet aggregation.

Tabuchi A, Yoshioka A, Higashi T, Shirakawa R, Nishioka H, Kita T, Horiuchi H

The Journal of biological chemistry   278 ( 29 )   26374 - 26379   2003.7（ ISSN:0021-9258 ）

Molecular dissection of alpha- and dense-core granule secretion of platelets.

Yoshioka A, Horiuchi H, Shirakawa R, Nishioka H, Tabuchi A, Higashi T, Yamamoto A, Kita T

Annals of the New York Academy of Sciences   947   403 - 406   2001.12（ ISSN:0077-8923 ）

Identification of protein kinase Calpha as an essential, but not sufficient, cytosolic factor for Ca2+-induced alpha- and dense-core granule secretion in platelets.

Yoshioka A, Shirakawa R, Nishioka H, Tabuchi A, Higashi T, Ozaki H, Yamamoto A, Kita T, Horiuchi H

The Journal of biological chemistry   276 ( 42 )   39379 - 39385   2001.10（ ISSN:0021-9258 ）

Remodeling of cell-cell junctions during cytokinesis in the vertebrate epithelium

ASCB Annual Meeting (PA, USA)  

Analysis of LSR, ILDR1 and ILDR2: Tricellulin recruitment, epithelial barrier function and implication in deafness pathogenesis.

TJ conference (Merida, Mexico)