ジーンファクトリー部門

活動内容

患者さんの組織や血液、他部門で加工・創出される生体材料（がん組織由来培養細胞、担がん動物等）および世界中で入手可能な市販の生体由来材料における核酸塩基配列情報の取得・解析を行うことにより、各種疾患の病気の原因となる遺伝子や薬の効果等に関係する遺伝子の探索、評価をします。

また、生物種によらず幅広い検体についての核酸塩基配列情報の取得・解析も行います。

スタッフ

家村　俊一郎	教授
磯貝　隆夫	特任教授

主な論文・学会発表等

研究員の過去の主な業績をリストアップしています

データベース

FLJ Human cDNA Database, http://flj.lifesciencedb.jp/

磯貝らは、FLJヒト完全長cDNAの取得・配列解析とそのデータベースを構築した

論文

Isogai, T., & Wakamatsu, A. Development of DNA probes to analyze the human mRNA diversity using data of FLJ human cDNAs. AGBT2013 (The 14th Annual Advances in Genome Biology and Technology), Marco Island, FL, USA , Feb. 20-23 (2013)

Wakamatsu, A., Imai, J., Watanabe, S., Isogai, T. Alternative splicing of genes during neuronal differentiation of NT2 pluripotential human embryonal carcinoma cells. FEBS Lett, 584, 4041-4047 (2010)
Wakamatsu, A., Kimura, K., Yamamoto, J., Nishikawa, T., Nomura, N., Sugano, S., Isogai, T. Identification and Functional Analyses of 11,769 Full-length Human cDNAs Focused on Alternative Splicing. DNA Res, 16, 371-383 (2009)
Kimura, K., Wakamatsu, A., Suzuki, Y., Ota, T., Nishikawa, T., Yamashita, R., Yamamoto, J., Sekine, M., Tsuritani, K., Wakaguri, H., Ishii, S., Sugiyama, T., Saito, K., Isono, Y., Irie, R., Kushida, N., Yoneyama, T., Otsuka, R., Kanda, K., Yokoi, T., Kondo, H., Wagatsuma, M., Murakawa, K., Ishida, S., Ishibashi, T., Takahashi-Fujii, A., Tanase, T., Nagai, K., Kikuchi, H., Nakai, K., Isogai, T., Sugano, S. Diversification of transcriptional modulation: Large-scale identification and characterization of putative alternative promoters of human genes. Genome Res., 16, 55-65 (2006)
Otsuki, T., Ota, T., Nishikawa, T., Hayashi, K., Suzuki, Y., Yamamoto, J., Wakamatsu, A., Kimura, K., Sakamoto, K., Hatano, N., Kawai, Y., Ishii, S., Saito, K., Kojima, S., Sugiyama, T., Ono, T., Okano, K., Yoshikawa, Y., Aotsuka, S., Sasaki, N., Hattori, A., Okumura, K., Nagai, K., Sugano, S., Isogai, T. Signal Sequence and Keyword Trap in silico for Selection of Full-Length Human cDNAs Encoding Secretion or Membrane Proteins from Oligo-Capped cDNA Libraries. DNA Res., 12: 117-126 (2005)
Ota, T., Suzuki, Y., Nishikawa, T., Otsuki, T., Sugiyama, T., Irie, R., Wakamatsu, A., Hayashi, K., Sato, H., Nagai, K., Kimura, K., Makita, H., Sekine, M., Obayashi, M., Nishi, T., Shibahara, T., Tanaka, T., Ishii, S., Yamamoto, J., Saito, K., Kawai, Y., Isono, Y., Nakamura, Y., Nagahari, K., Murakami, K., Yasuda, T., Iwayanagi, T., Wagatsuma, M., Shiratori, A., Sudo, H., Hosoiri, T., Kaku, Y., Kodaira, H., Kondo, H., Sugawara, M., Takahashi, M., Kanda, K., Yokoi, T., Furuya, T., Kikkawa, E., Omura, Y., Abe, K., Kamihara, K., Katsuta, N., Sato, K., Tanikawa, M., Yamazaki, M., Ninomiya, K., Ishibashi, T., Yamashita, H., Murakawa, K., Fujimori, K., Tanai, H., Kimata, M., Watanabe, M., Hiraoka, S., Chiba, Y., Ishida, S., Ono, Y., Takiguchi, S., Watanabe, S., Yosida, M., Hotuta, T., Kusano, J., Kanehori, K., Takahashi-Fujii, A., Hara, H., Tanase, TO., Nomura, Y., Togiya, S., Komai, F., Hara, R., Takeuchi, K., Arita, M., Imose, N., Musashino, K., Yuuki, H., Oshima, A., Sasaki, N., Aotsuka, S., Yoshikawa, Y., Matsunawa, H., Ichihara, T., Shiohata, N., Sano, S., Moriya, S., Momiyama, H., Satoh, N., Takami, S., Terashima, Y., Suzuki, O., Nakagawa, S., Senoh, A., Mizoguchi, H., Goto, Y., Shimizu, F., Wakebe, H., Hishigaki, H., Watanabe, T., Sugiyama, A., Takemoto, M., Kawakami, B., Yamazaki, M., Watanabe, K., Kumagai, A., Itakura, S., Fukuzumi, Y., Fujimori, Y., Komiyama, M., Tashiro, H., Tanigami, A., Fujiwara, T., Ono, T., Yamada, K., Fujii, Y., Ozaki, K., Hirao, M., Ohmori, Y., Kawabata, A., Hikiji, T., Kobatake, N., Inagaki, H., Ikema, Y., Okamoto, S., Okitani, R., Kawakami, T., Noguchi, S., Itoh, T., Shigeta, K., Senba, T., Matsumura, K., Nakajima, Y., Mizuno, T., Morinaga, M., Sasaki, M., Togashi, T., Oyama, M., Hata, H., Watanabe, M., Komatsu, T., Mizushima-Sugano, J., Satoh, T., Shirai, Y., Takahashi, Y., Nakagawa, K., Okumura, K., Nagase, T., Nomura, N., Kikuchi, H., Masuho, Y., Yamashita, R., Nakai, K., Yada, T., Nakamura, Y., Ohara, O., Isogai, T., Sugano, S. Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet, 36, 40-45 (2004)

著書

Isogai, T., & Wakamatsu, A. mRNA diversity of genes by alternative splicing during neuronal differentiation of NT2 pluripotential human embryonal carcinoma cells by all-trans retinoic acid. Editors: Cheng, L-H., & Yuto, Ito, Y. (2012). “Retinoic Acid: Structure, Mechanisms and Roles in Disease”, Nova Science Publishers, Inc. Hauppauge, NY, USA. 53-86 (2012) ISBN: 978-1-62100-597-1_ch3 (Open Access)
https://www.novapublishers.com/catalog/product_info.php?products_id=34516

学会発表

Isogai, T., & Wakamatsu, A. Development of DNA probes to analyze the human mRNA diversity using data of FLJ human cDNAs. AGBT2013 (The 14th Annual Advances in Genome Biology and Technology), Marco Island, FL, USA , Feb. 20-23 (2013)